foreign protein

Synthesizing protein by the mammary gland bioreactor is the hot research areas in biotechnology, but specifically and efficiently expression of foreign protein in mammary gland is influenced by many factors, needing more research.

用乳腺生物反应器制备目的药用蛋白是目前生物技术研究的热点，但外源蛋白在乳腺组织中特异、高效表达受诸多因素影响，仍需进一步研究。

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Yeast is a kind of eukaryotic expression system, which has been widely used in recent years. It is not only convenient to purification, but also can promote the secretion and expression efficacy of foreign protein by using signal peptide sequence to construct yeast secretory expression system.

酵母是一个近年来被广泛使用的真核表达系统，应用信号肽序列构建酵母分泌表达系统，即可方便纯化操作，又可提高外源蛋白的分泌表达效率。

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RT-PCR was used to detect the transcription of TGEV spike protein, enhanced green fluorescence ( eGFP) was used to test the expression of foreign protein, and serial passage and PCR were used to detect the stability and safety of rAd-S.

以RT-PCR法检测重组腺病毒rAd-S中TGEV纤突糖蛋白S基因的转录，增强型绿色荧光蛋白（eGFP）荧光检测外源基因的表达，用连续传代和PCR检测rAd-S的稳定性和安全性。

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The followed approach to analyze the properties of the recombinant strain, and used for surface display other functional foreign protein is under the way.

对重组菌性能的分析测定、以及进一步用于展示其它功能性外源蛋白的工作仍然在进行中。

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To practice the multi-variable analysis of the factors that may affect the growth and the foreign protein expression of engineered E. coli in shake-flask scale and confirm how these factors affect the growth and protein expression.

在摇瓶水平上对可影响大肠杆菌生长及外源蛋白表达的因素进行多因素分析，明确这些影响因素对菌体生长及表达的影响程度。

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Since the urate oxidase in human body does not have activity, the current clinical application of urate oxidase is mainly rooted from Aspergillus flavus. Since it is a foreign protein with immunogenicity, clinical application is greatly limited.

由于人体内的尿酸氧化酶不具有活性，目前临床上用的尿酸氧化酶主要来自黄曲霉菌（aspergillus flavus），因其是外源蛋白，具有免疫原性，临床应用受到极大限制。

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Construction of Expression Vector of Foreign Protein Locating Potato Starch Grain

外源蛋白定位马铃薯淀粉粒表达载体的构建

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Strong favorable tool for foreign protein expression-Pichia pastoris

表达外源蛋白的有利工具-毕赤酵母表达系统

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Advance in the Expression of Foreign Protein in Pichia Pastoris

毕赤巴斯德酵母表达外源蛋白研究进展

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Diphtheria antitoxin is a foreign protein.

白喉抗毒素是异性蛋白.

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Baculovirus is a kind of double-stranded circle large DNA virus, which has been widely developed as biopesticides and a powerful vector of foreign protein expressions.

杆状病毒是一类环状双链DNA病毒，其作为一种安全的生物杀虫剂以及外源蛋白的高效表达载体，已经在世界范围内得到了广泛的应用。

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Expression in Plants of Foreign Protein Mediated by Tobacco Mosaic Virus

TMV介导的外源蛋白在植物中表达

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The plant's foreign protein expression system has made a great progress.

植物外源蛋白表达系统已取得较大进展。

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The data showed that N source stabilized the expression of foreign protein and C source altered the synthesis of cell walls.

氮源能稳定外源蛋白的表达，而碳源则影响细胞壁的合成。

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Anaphylactic shock is the reaction of the body overwhelming sensitization by a foreign protein.

过敏性休克是机体对异种蛋白高度敏感的一种反应.

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The result indicate that the anchor efficiency was variance obviously with different anchor system even in the same host and the same foreign protein.

以上结果说明相同宿主，相同外源蛋白，不同锚定系统的锚定效率有明显差异。

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Several Critical Problems Need to be Resolved in Foreign Protein Expression using Plants

利用植物表达外源蛋白需解决的几个关键问题

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Objective: To reduce acetate accumulation in the culture of Escherichia coli by utilizing phosphotransacetylase ( PTA) mutant, so as to promote foreign protein expression and facilitate high cell density cultivation.

目的：利用大肠杆菌磷酸转乙酰基酶（PTA）缺陷变株减少工程菌乙酸的产生，以利于工程菌高密度培养及外源蛋白表达。

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Proteomic Characterization of Human Erythrocyte 20S Proteasome and Analysis of Species-dependent 20S Proteasome Heterogeneity Diphtheria antitoxin is a foreign protein.

人红细胞20S蛋白酶体的蛋白质组学表征及不同来源20S蛋白酶体异质性的研究白喉抗毒素是异性蛋白。

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The effects of signal peptide on efficiency of foreign protein secretion

信号肽对外源蛋白分泌效率的影响

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There was no difference between human/ mouse cell. CONCLUSION: Human embryonic fibroblast cells can be used to support proliferation of human embryonic stem cells, eliminating the influence of foreign protein.

结论：完全可以使用人胚胎成纤维细胞支持人胚胎干细胞增殖，消除异种蛋白污染的可能性，为胚胎干细胞定向诱导分化发育应用于临床打下坚实基础。

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The Transgenic Plants Express Foreign Medical Protein

转基因植物表达外源药用蛋白的研究进展

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The article reviews some aspects on generation and characterization of mammary gland bioreactor, separation and purification of foreign protein from milk and some questions that need to be answered on the route.

本文仅就动物乳腺生物反应器的建立、检测、目的蛋白的分离纯化以及存在的问题等作一综述。

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Conclusion: The way for the purification of foreign protein by inserting His Tag gene into vaccinia virus vectors was reasonable and using fluorescence protein as a selecting marker should be researched further.

结论：将His-Tag的纯化标签加入到通用型痘苗病毒表达载体中用于纯化表达的目的蛋白，该方法是可行的.荧光蛋白基因作为一个筛选标记基因应用于痘苗病毒载体系统还有待进行进一步研究。

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Advance in improving expression of foreign protein in plant

增强异源蛋白在植物体内表达的研究进展

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Conclusion: The GAP promoter can be used to express foreign protein effectively and is the basis for studying the physicochemical properties and genetic mechanism of Gassericin T.

结论：为下一步在毕赤酵母中组成型表达外源蛋白，研究其作用机理和遗传机制奠定了基础。

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Pichia pastoris is a single-celled eukaryotes the past 10 years, as was the eukaryotic expression system for foreign protein production.

毕赤酵母是一种单细胞真核生物，近十年来被作为真核表达系统而用于外源蛋白质的生产。

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Methods The expression of the foreign protein in the obtained transgenic potatoes was assayed by ELISA.

方法在根癌脓杆菌介导获得系列转基因马铃薯植株的基础上，用ELISA分析转基因马铃薯中目的蛋白表达水平。

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A.Christmann did a lot of tests and have successfully integrated foreign protein into plasmid, which was detected by sensitive fluorescent immune detection technology.

A.Christmann等做了大量实验，已成功将外源片段整合到质粒中，并利用灵敏的荧光免疫检测技术检测到。

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While adaptive immune responses require prior exposure to a foreign protein such as a virus or bacterium, innate immunity does not.

获得性免疫应答需要事先暴露于外界蛋白质，如病毒或细菌。先天性免疫不需要。

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